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UniProtKB/Swiss-Prot entry O88844

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name IDHC_MOUSE
Primary accession number O88844
Secondary accession numbers None
Integrated into Swiss-Prot on May 30, 2000
Sequence was last modified on November 1, 1998 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 66)
Name and origin of the protein
Protein name Isocitrate dehydrogenase [NADP] cytoplasmic
Synonyms IDH
EC 1.1.1.42
Cytosolic NADP-isocitrate dehydrogenase
Oxalosuccinate decarboxylase
NADP(+)-specific ICDH
IDP
Gene name
Name: Idh1
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
PubMed=9866202 [NCBI, ExPASy, EBI, Israel, Japan]
Nekrutenko A., Hillis D.M., Patton J.C., Bradley R.D., Baker R.J.;
"Cytosolic isocitrate dehydrogenase in humans, mice, and voles and phylogenetic analysis of the enzyme family.";
Mol. Biol. Evol. 15:1674-1684(1998).
[2]
 PROTEIN SEQUENCE OF 30-49.
TISSUE=Brain;
Lubec G., Yang J.W., Zigmond M.;
Submitted (JUL-2007) to UniProtKB.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF020039; AAD02919.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
UniGene Mm.9925
3D structure databases
PDB
2CMJ; X-ray; 1.99 A; A/B=4-413.[ExPASy / RCSB / EBI]
2CMV; X-ray; 2.52 A; A/B=4-413.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 2CMJ; -.
2CMV; -.
ModBase O88844.
PTM databases
PhosphoSite O88844; -.
2D gel databases
SWISS-2DPAGE O88844; -.
REPRODUCTION-2DPAGE O88844; -.
Organism-specific databases
MGI MGI:96413; Idh1.
Gene expression databases
ArrayExpress O88844; -.
CleanEx MM_IDH1; -.
GermOnline ENSMUSG00000025950; Mus musculus.
Ontologies
GO
GO:0005829; Cellular component: cytosol (inferred from direct assay from MGI).
GO:0004450; Molecular function: isocitrate dehydrogenase (NADP+) activity (inferred from direct assay from MGI).
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0030145; Molecular function: manganese ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006749; Biological process: glutathione metabolic process (inferred from mutant phenotype from MGI).
GO:0006097; Biological process: glyoxylate cycle (inferred from electronic annotation from UniProtKB-KW).
GO:0006102; Biological process: isocitrate metabolic process (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0006979; Biological process: response to oxidative stress (inferred from direct assay from MGI).
GO:0006099; Biological process: tricarboxylic acid cycle (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR004790; IsoCit_DHase_NADP-dep_euk.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.718.10; IDH_IMDH; 1.
PANTHER PTHR11822; IDH_NADP_euk; 1.
Pfam PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000108; IDH_NADP; 1.
TIGRFAMs TIGR00127; nadp_idh_euk; 1.
PROSITE PS00470; IDH_IMDH; 1.
ProtoNet O88844.
Genome annotation databases
Ensembl ENSMUSG00000025950; Mus musculus. [Contig view]
Phylogenomic databases
HOVERGEN O88844; -.
Other
SOURCE Idh1; Mus musculus.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Cytoplasm; Direct protein sequencing; Glyoxylate bypass; Magnesium; Manganese; Metal-binding; NADP; Oxidoreductase; Tricarboxylic acid cycle.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   414  414     Isocitrate dehydrogenase [NADP] cytoplasmic. PRO_0000083578
NP_BIND   75    77  3     NADP (By similarity). 
NP_BIND   310   315  6     NADP (By similarity). 
REGION   94   100  7     Substrate binding (By similarity). 
METAL   252   252        Magnesium or manganese (By similarity). 
METAL   275   275        Magnesium or manganese (By similarity). 
BINDING   77    77        Substrate (By similarity). 
BINDING   82    82        NADP (By similarity). 
BINDING   109   109        Substrate (By similarity). 
BINDING   132   132        Substrate (By similarity). 
BINDING   260   260        NADP (By similarity). 
BINDING   328   328        NADP; via amide nitrogen and carbonyl oxygen (By similarity). 
SITE   139   139  1     Critical for catalysis (By similarity). 
SITE   212   212  1     Critical for catalysis (By similarity). 
STRAND   5    14  10      
HELIX   17    30  14      
TURN   31    34  4      
STRAND   35    43  9      
HELIX   46    51  6      
TURN   52    54  3      
HELIX   55    67  13      
STRAND   68    72  5      
HELIX   80    86  7      
HELIX   95   103  9      
STRAND   106   111  6      
STRAND   128   133  6      
HELIX   137   140  4      
STRAND   142   146  5      
STRAND   148   162  15      
STRAND   165   172  8      
STRAND   177   185  9      
HELIX   186   203  18      
STRAND   207   211  5      
TURN   213   215  3      
HELIX   219   234  16      
HELIX   236   241  6      
STRAND   246   250  5      
HELIX   251   260  10      
STRAND   265   269  5      
HELIX   271   285  15      
STRAND   290   296  7      
STRAND   303   306  4      
HELIX   313   320  8      
HELIX   330   347  18      
HELIX   350   368  19      
HELIX   374   381  8      
HELIX   383   385  3      
HELIX   388   390  3      
HELIX   394   412  19      
Sequence information
Length: 414 AA [This is the length of the unprocessed precursor] Molecular weight: 46660 Da [This is the MW of the unprocessed precursor] CRC64: AA482EA1C4114CAD [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MSRKIQGGSV VEMQGDEMTR IIWELIKEKL ILPYVELDLH SYDLGIENRD ATNDQVTKDA 

        70         80         90        100        110        120 
AEAIKKYNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR NILGGTVFRE AIICKNIPRL 

       130        140        150        160        170        180 
VTGWVKPIII GRHAYGDQYR ATDFVVPGPG KVEITYTPKD GTQKVTYMVH DFEEGGGVAM 

       190        200        210        220        230        240 
GMYNQDKSIE DFAHSSFQMA LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKKYKSQFE 

       250        260        270        280        290        300 
AQNICYEHRL IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLICPDG 

       310        320        330        340        350        360 
KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWSRGL AHRAKLDNNT ELSFFAKALE 

       370        380        390        400        410 
DVCIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK LGENLKAKLA QAKL
O88844 in FASTA format

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