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[1]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-14, AND SUBUNIT.
STRAIN=DSM 6984 / K172;
DOI=10.1046/j.1432-1327.1998.2560148.x; PubMed=9746358 [NCBI, ExPASy, EBI, Israel, Japan]
Breese K.,
Boll M.,
Alt-Moerbe J.,
Schaegger H.,
Fuchs G.;
"Genes coding for the benzoyl-CoA pathway of anaerobic aromatic metabolism in the bacterium Thauera aromatica.";
Eur. J. Biochem. 256:148-154(1998).
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[2]
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CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, AND COFACTOR.
STRAIN=DSM 6984 / K172;
DOI=10.1111/j.1432-1033.1995.921_a.x; PubMed=8575453 [NCBI, ExPASy, EBI, Israel, Japan]
Boll M.,
Fuchs G.;
"Benzoyl-coenzyme A reductase (dearomatizing), a key enzyme of anaerobic aromatic metabolism. ATP dependence of the reaction, purification and some properties of the enzyme from Thauera aromatica strain K172.";
Eur. J. Biochem. 234:921-933(1995).
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[3]
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CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
STRAIN=DSM 6984 / K172;
DOI=10.1128/JB.183.3.968-979.2001; PubMed=11208796 [NCBI, ExPASy, EBI, Israel, Japan]
Laempe D.,
Jahn M.,
Breese K.,
Schaegger H.,
Fuchs G.;
"Anaerobic metabolism of 3-hydroxybenzoate by the denitrifying bacterium Thauera aromatica.";
J. Bacteriol. 183:968-979(2001).
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- FUNCTION: Catalyzes the anaerobic reduction of benzoyl-CoA and 3-hydroxybenzoyl-CoA to form cyclohexa-1,5-diene-1-carbonyl-CoA and 3-hydroxycyclohexa-1,5-diene-1-carbonyl-CoA, respectively. The enzyme also reduces other benzoyl-CoA analogs with small substituents at the aromatic ring.
- CATALYTIC ACTIVITY: Benzoyl-CoA + reduced acceptor + 2 ATP = cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + 2 ADP + 2 phosphate.
- CATALYTIC ACTIVITY: 3-hydroxybenzoyl-CoA + reduced acceptor + 2 ATP = 3-hydroxycyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + 2 ADP + 2 phosphate.
- COFACTOR: Iron-sulfur. May be a 4Fe-4S cluster.
- BIOPHYSICOCHEMICAL PROPERTIES:
- SUBUNIT: Heterotetramer composed of A, B, C, and D subunits.
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Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 282 AA [This is the length of the unprocessed precursor] |
Molecular weight: 30157 Da [This is the MW of the unprocessed precursor] |
CRC64: 248D3D6305C90514 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MTITAGIDIG TGAVKTVLFR VEGDKTEWLA KRNDRIRQRD PFKLAEEAYN GLLEEAGLKA
70 80 90 100 110 120
SDVDYVATTG EGESLAFHTG HFYSMTTHAR GAVYLNPEAR AVLDIGALHG RAIRNDERGK
130 140 150 160 170 180
VETYKMTSQC ASGSGQFLEN IARYLGIAQD EIGSLSTQAD NPEVVSSICA VLAETDVINM
190 200 210 220 230 240
VSRGISAPNI LKGIHISMAG RLAKLLKSVG ARDGVVLCTG GLALDEGLLK TLNESIQEQK
250 260 270 280
MAVVAYNHPD SPYAGAIGAA LWGAFRHEKL ARLGQQQVAE AA
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O87877 in FASTA format |
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