ID   AP1B1_MOUSE             Reviewed;         943 AA.
AC   O35643; Q922E2;
DT   20-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   04-NOV-2008, entry version 72.
DE   RecName: Full=AP-1 complex subunit beta-1;
DE   AltName: Full=Adapter-related protein complex 1 subunit beta-1;
DE   AltName: Full=Adaptor protein complex AP-1 subunit beta-1;
DE   AltName: Full=Beta-adaptin 1;
DE   AltName: Full=Beta1-adaptin;
DE   AltName: Full=Golgi adaptor HA1/AP1 adaptin beta subunit;
DE   AltName: Full=Clathrin assembly protein complex 1 beta large chain;
GN   Name=Ap1b1; Synonyms=Adtb1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=BALB/c; TISSUE=Embryonic brain;
RX   MEDLINE=97419266; PubMed=9271666; DOI=10.1007/s003359900531;
RA   Guilbaud C., Peyrard M., Fransson I., Clifton S.W., Roe B.A.,
RA   Carter N.P., Dumanski J.P.;
RT   "Characterization of the mouse beta-prime adaptin gene; cDNA sequence,
RT   genomic structure, and chromosomal localization.";
RL   Mamm. Genome 8:651-656(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Mammary gland;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   NITRATION [LARGE SCALE ANALYSIS] AT TYR-574, AND MASS SPECTROMETRY.
RC   TISSUE=Brain;
RX   PubMed=16800626; DOI=10.1021/bi060474w;
RA   Sacksteder C.A., Qian W.-J., Knyushko T.V., Wang H., Chin M.H.,
RA   Lacan G., Melega W.P., Camp D.G. II, Smith R.D., Smith D.J.,
RA   Squier T.C., Bigelow D.J.;
RT   "Endogenously nitrated proteins in mouse brain: links to
RT   neurodegenerative disease.";
RL   Biochemistry 45:8009-8022(2006).
CC   -!- FUNCTION: Subunit of clathrin-associated adaptor protein complex 1
CC       that plays a role in protein sorting in the late-Golgi/trans-Golgi
CC       network (TGN) and/or endosomes. The AP complexes mediate both the
CC       recruitment of clathrin to membranes and the recognition of
CC       sorting signals within the cytosolic tails of transmembrane cargo
CC       molecules.
CC   -!- SUBUNIT: Adaptor protein complex 1 (AP-1) is an heterotetramer
CC       composed of two large adaptins (gamma-type subunit AP1G1 and beta-
CC       type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or
CC       AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or
CC       AP1S3).
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus. Cytoplasmic vesicle,
CC       clathrin-coated vesicle membrane; Peripheral membrane protein;
CC       Cytoplasmic side (By similarity). Golgi apparatus (By similarity).
CC       Note=Component of the coat surrounding the cytoplasmic face of
CC       coated vesicles located at the Golgi complex (By similarity).
CC   -!- TISSUE SPECIFICITY: Widely expressed.
CC   -!- SIMILARITY: Belongs to the adaptor complexes large subunit family.
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DR   EMBL; Y07919; CAA69224.1; -; mRNA.
DR   EMBL; BC008513; AAH08513.1; -; mRNA.
DR   UniGene; Mm.274816; -.
DR   SMR; O35643; 711-943.
DR   PhosphoSite; O35643; -.
DR   Ensembl; ENSMUSG00000009090; Mus musculus.
DR   MGI; MGI:1096368; Ap1b1.
DR   HOVERGEN; O35643; -.
DR   LinkHub; O35643; -.
DR   ArrayExpress; O35643; -.
DR   CleanEx; MM_AP1B1; -.
DR   GO; GO:0005802; C:trans-Golgi network; TAS:MGI.
DR   GO; GO:0005515; F:protein binding; IPI:UniProtKB.
DR   GO; GO:0006886; P:intracellular protein transport; TAS:MGI.
DR   GO; GO:0016192; P:vesicle-mediated transport; TAS:MGI.
DR   InterPro; IPR016342; AP_complex_bsu.
DR   InterPro; IPR012295; b_Adaptin_TBP_C.
DR   InterPro; IPR002553; Clathrin/coatomer_adapt-like_N.
DR   InterPro; IPR008152; Clathrin_a/b/g-adaptin_app_Ig.
DR   InterPro; IPR013037; Clathrin_b-adaptin_app_Ig-like.
DR   InterPro; IPR015151; Clathrin_b-adaptin_app_sub_C.
DR   Gene3D; G3DSA:3.30.310.10; b_Adaptin_TBP_C; 1.
DR   Gene3D; G3DSA:2.60.40.1150; Clathrin_b-adaptin_app_Ig-like; 1.
DR   Pfam; PF01602; Adaptin_N; 1.
DR   Pfam; PF02883; Alpha_adaptinC2; 1.
DR   Pfam; PF09066; B2-adapt-app_C; 1.
DR   PIRSF; PIRSF002291; AP_complex_beta; 1.
DR   SMART; SM00809; Alpha_adaptinC2; 1.
PE   1: Evidence at protein level;
KW   Cytoplasmic vesicle; Endocytosis; Golgi apparatus; Membrane;
KW   Nitration; Protein transport; Transport.
FT   CHAIN         1    943       AP-1 complex subunit beta-1.
FT                                /FTId=PRO_0000193739.
FT   COMPBIAS    576    722       Pro-rich (stalk region).
FT   MOD_RES     574    574       Nitrated tyrosine.
FT   CONFLICT    227    227       D -> A (in Ref. 2; AAH08513).
FT   CONFLICT    288    288       L -> P (in Ref. 2; AAH08513).
SQ   SEQUENCE   943 AA;  103979 MW;  7A67869C1CAAF973 CRC64;
     MTDSKYFTTT KKGEIFELKA ELNSDKKEKK KEAVKKVIAS MTVGKDVSAL FPDVVNCMQT
     DNLELKKLVY LYLMNYAKSQ PDMAIMAVNT FVKDCEDPNP LIRALAVRTM GCIRVDKITE
     YLCEPLRKCL KDEDPYVRKT AAVCVAKLHD INAQLVEDQG FLDTLKDLIS DSNPMVVANA
     VAALSEIAES HPSSNLLDLN PQSINKLLTA LNECTEWGQI FILDCLDNYM PKDDREAQSI
     CERVTPRLSH ANSAVVLSAV KVLMKFMEML SKDLDYYATL LKKLAPPLVT LLSAEPELQY
     VALRNINLIV QKRPEILKHE MKVFFVKYND PIYVKLEKLD IMIRLASQAN IAQVLAELKE
     YATEVDVDFV RKAVRAIGRC AIKVEQSAER CVSTLLDLIQ TKVNYVVQEA IVVIKDIFRK
     YPNKYESVIA TLCENLDSLD EPEARAAMIW IVGEYAERID NADELLESFL EGFHDESTQV
     QLQLLTAIVK LFLKKPTETQ ELVQQVLSLA TQDSDNPDLR DRGYIYWRLL STDPVAAKEV
     VLAEKPLISE ETDLIEPTLL DELICYIGTL ASVYHKPPNA FVEGGRGVVH KSLPPRTASS
     ESTESPETAP AGAPAGDQPD VIPAQGDLLG DLLNLDLGPP VSGPPLAASS VQMGAVDLLG
     GGLDSLIGDS NFGAPSASVA AAPAPARLGA PISSGLSDLF DLTSGVGTLS GSYVAPKAVW
     LPAMKAKGLE ISGTFTRQAG SISMDLQLTN KALQVMTDFA IQFNRNSFGL APAAPLQVHV
     PLSPNQTVEI SLPLNTVGSV LKMEPLNNLQ VAVKNNIDVF YFSTLYPLHV LFVEDGKMDR
     QMFLATWKDI ANENEAQFQI RDCPLNTEAA SNKLQSSNIF TVAKRNVEGQ DMLYQSLKLT
     NGIWVLAELR IQPGNPSFTL SLKCRAPEVS QHVYQAYETI LKN
//