[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY. STRAIN=BALB/c; PubMed=9427309 [NCBI, ExPASy, EBI, Israel, Japan] Naciff J.M., Misawa H., Dedman J.R.; "Molecular characterization of the mouse vesicular acetylcholine transporter gene."; NeuroReport 8:3467-3473(1997).

Comments

FUNCTION: Involved in acetylcholine transport into synaptic vesicles (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in the spinal cord, brain (excluding the cerebellum), brain stem and cholinergic tissues. Not expressed in peripheral tissues such as liver and kidney.
SIMILARITY: Belongs to the major facilitator superfamily. Vesicular transporter family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF019045; AAD09151.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

IPI

IPI00129630; -.

UniGene

Mm.190503

3D structure databases

ModBase

O35304.

PTM databases

PhosphoSite

O35304; -.

Organism-specific databases

MGI

MGI:1101061; Slc18a3.

Ontologies

GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0043195;	Cellular component: terminal button (inferred from direct assay from MGI).
GO:0008504;	Molecular function: monoamine transmembrane transporter activity (traceable author statement from MGI).
GO:0006836;	Biological process: neurotransmitter transport (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR011701; MFS_1.
Graphical view of domain structure.

Pfam

PF07690; MFS_1; 1.
Pfam graphical view of domain structure.

PROSITE

PS50850; MFS; 1.
PROSITE graphical view of domain structure (profiles).

Phylogenomic databases

HOVERGEN

O35304; -.

Other

SOURCE

Slc18a3; Mus musculus.

ProtoNet

O35304.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Glycoprotein; Membrane; Neurotransmitter transport; Transmembrane; Transport.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 530`	`530`	`Vesicular acetylcholine transporter.`	`PRO_0000127519`
`TOPO_DOM`	`1 33`	`33`	`Cytoplasmic (Potential).`
`TRANSMEM`	`34 54`	`21`	`Potential.`
`TOPO_DOM`	`55 125`	`71`	`Lumenal, vesicle (Potential).`
`TRANSMEM`	`126 146`	`21`	`Potential.`
`TOPO_DOM`	`147 152`	`6`	`Cytoplasmic (Potential).`
`TRANSMEM`	`153 173`	`21`	`Potential.`
`TOPO_DOM`	`174 182`	`9`	`Lumenal, vesicle (Potential).`
`TRANSMEM`	`183 203`	`21`	`Potential.`
`TOPO_DOM`	`204 213`	`10`	`Cytoplasmic (Potential).`
`TRANSMEM`	`214 234`	`21`	`Potential.`
`TOPO_DOM`	`235 242`	`8`	`Lumenal, vesicle (Potential).`
`TRANSMEM`	`243 263`	`21`	`Potential.`
`TOPO_DOM`	`264 288`	`25`	`Cytoplasmic (Potential).`
`TRANSMEM`	`289 309`	`21`	`Potential.`
`TOPO_DOM`	`310 325`	`16`	`Lumenal, vesicle (Potential).`
`TRANSMEM`	`326 346`	`21`	`Potential.`
`TOPO_DOM`	`347 356`	`10`	`Cytoplasmic (Potential).`
`TRANSMEM`	`357 377`	`21`	`Potential.`
`TOPO_DOM`	`378 388`	`11`	`Lumenal, vesicle (Potential).`
`TRANSMEM`	`389 409`	`21`	`Potential.`
`TOPO_DOM`	`410 422`	`13`	`Cytoplasmic (Potential).`
`TRANSMEM`	`423 443`	`21`	`Potential.`
`TOPO_DOM`	`444 447`	`4`	`Lumenal, vesicle (Potential).`
`TRANSMEM`	`448 468`	`21`	`Potential.`
`TOPO_DOM`	`469 530`	`62`	`Cytoplasmic (Potential).`
`CARBOHYD`	`89 89`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`96 96`		`N-linked (GlcNAc...) (Potential).`

Sequence information

Length: 530 AA [This is the length of the unprocessed precursor]

Molecular weight: 56615 Da [This is the MW of the unprocessed precursor]

CRC64: 08526F29B4E8EEDD [This is a checksum on the sequence]

        10         20         30         40         50         60 
MEPTAPTGQA RAAATKLSEA VGAALQEPQR QRRLVLVIVC VALLLDNMLY MVIVPIVPDY 

        70         80         90        100        110        120 
IAHMRGGSES PTLISEVWEP TLPPPTLANA SAYLANTSAS PTAAGSARSI LRPRYPTESE 

       130        140        150        160        170        180 
DVKIGVLFAS KAILQLLVNP LSGPFIDRMS YDVPLLIGLG VMFASTVMFA FAEDYATFFA 

       190        200        210        220        230        240 
ARSLQGLGSA FADTSGIAMI ADKYPEEPER SRALGVALAF ISFGSLVAPP FGGILYEFAG 

       250        260        270        280        290        300 
KRVPFLVLAA VSLFDALLLL AVAKPFSAAA RARANLPVGT PIHRLMLDPY IAVVAGALTT 

       310        320        330        340        350        360 
CNIPLAFLEP TIATWMKHTM AASEWEMGMV WLPAFVPHVL GVYLTVRLAA RYPHLQWLYG 

       370        380        390        400        410        420 
ALGLAVIGVS SCVVPACRSF APLVVSLCGL CFGIALVDTA LLPTLAFLVD VRHVSVYGSV 

       430        440        450        460        470        480 
YAIADISYSV AYALGPIVAG HIVHSLGFEQ LSLGMGLANL LYAPVLLLLR NVGLLTRSRS 

       490        500        510        520        530 
ERDVLLDEPP QGLYDAVRLR EVQGKDGGEP CSPPGPFDGC EDDYNYYSRS

O35304 in FASTA format

View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools