ID   SKFA_BACSU              Reviewed;          55 AA.
AC   O31422;
DT   11-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   04-NOV-2008, entry version 44.
DE   RecName: Full=Sporulation-killing factor skfA;
GN   Name=skfA; Synonyms=ybcO; OrderedLocusNames=BSU01910;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Haga K., Liu H., Yasumoto K., Takahashi H., Yoshikawa H.;
RT   "Sequence analysis of the 70kb region between 17 and 23 degree of the
RT   Bacillus subtilis chromosome.";
RL   Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   INHIBITION OF XANTHOMONAS GROWTH.
RX   PubMed=11851812; DOI=10.1046/j.1365-2672.2001.01475.x;
RA   Lin D., Qu L.-J., Gu H., Chen Z.;
RT   "A 3.1-kb genomic fragment of Bacillus subtilis encodes the protein
RT   inhibiting growth of Xanthomonas oryzae pv. oryzae.";
RL   J. Appl. Microbiol. 91:1044-1050(2001).
RN   [4]
RP   FUNCTION.
RC   STRAIN=168 / PY79;
RX   PubMed=12817086; DOI=10.1126/science.1086462;
RA   Gonzalez-Pastor J.E., Hobbs E.C., Losick R.;
RT   "Cannibalism by sporulating bacteria.";
RL   Science 301:510-513(2003).
RN   [5]
RP   ROLE IN LIPASE PRODUCTION.
RC   STRAIN=168;
RX   PubMed=15812018; DOI=10.1128/AEM.71.4.1899-1908.2005;
RA   Westers H., Braun P.G., Westers L., Antelmann H., Hecker M.,
RA   Jongbloed J.D.H., Yoshikawa H., Tanaka T., van Dijl J.M., Quax W.J.;
RT   "Genes involved in SkfA killing factor production protect a Bacillus
RT   subtilis lipase against proteolysis.";
RL   Appl. Environ. Microbiol. 71:1899-1908(2005).
RN   [6]
RP   REPRESSION BY ABRB.
RX   PubMed=15687200; DOI=10.1128/JB.187.4.1357-1368.2005;
RA   Fujita M., Gonzalez-Pastor J.E., Losick R.;
RT   "High- and low-threshold genes in the Spo0A regulon of Bacillus
RT   subtilis.";
RL   J. Bacteriol. 187:1357-1368(2005).
RN   [7]
RP   INDUCTION.
RC   STRAIN=168;
RX   PubMed=16816204; DOI=10.1128/JB.00084-06;
RA   Allenby N.E.E., Watts C.A., Homuth G., Pragai Z., Wipat A., Ward A.C.,
RA   Harwood C.R.;
RT   "Phosphate starvation induces the sporulation killing factor of
RT   Bacillus subtilis.";
RL   J. Bacteriol. 188:5299-5303(2006).
RN   [8]
RP   REPRESSION BY ABRB AND ABH.
RX   PubMed=17720793; DOI=10.1128/JB.01081-07;
RA   Strauch M.A., Bobay B.G., Cavanagh J., Yao F., Wilson A.,
RA   Le Breton Y.;
RT   "Abh and AbrB control of Bacillus subtilis antimicrobial gene
RT   expression.";
RL   J. Bacteriol. 189:7720-7732(2007).
CC   -!- FUNCTION: Induces the lysis of other B.subtilis cells that have
CC       not entered the sporulation pathway, providing a source of
CC       nutrients to support sporulation. Can also inhibit growth of other
CC       bacteria at high concentrations. Has a role in protecting the
CC       secreted lipase lipA against proteolysis, either by modulating its
CC       folding or by acting as a protease inhibitor.
CC   -!- SUBCELLULAR LOCATION: Secreted. Note=Secreted by the ABC
CC       transporter skfEF (Probable).
CC   -!- INDUCTION: By spo0A and phoP, during nutrient starvation,
CC       especially phosphate starvation. Repressed by abrB during normal
CC       growth when nutrients are plentiful, in association with the
CC       transcriptional repressor abh.
CC   -!- WEB RESOURCE: Name=Protein Spotlight; Note=I'll have you for
CC       supper - Issue 90 of January 2008;
CC       URL="http://www.expasy.org/spotlight/back_issues/sptlt090.shtml";
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DR   EMBL; AB006424; BAA33086.1; -; Genomic_DNA.
DR   EMBL; Z99105; CAB11984.1; -; Genomic_DNA.
DR   PIR; C69746; C69746.
DR   RefSeq; NP_388072.1; -.
DR   GeneID; 938506; -.
DR   GenomeReviews; AL009126_GR; BSU01910.
DR   KEGG; bsu:BSU01910; -.
DR   NMPDR; fig|224308.1.peg.191; -.
DR   SubtiList; BG12713; skfA.
DR   HOGENOM; O31422; -.
DR   BioCyc; BSUB224308:BSU0191-MON; -.
DR   LinkHub; O31422; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Antibiotic; Antimicrobial; Complete proteome; Secreted.
FT   CHAIN         1     55       Sporulation-killing factor skfA.
FT                                /FTId=PRO_0000049459.
SQ   SEQUENCE   55 AA;  5934 MW;  F169E627ADBEBB55 CRC64;
     MKRNQKEWES VSKKGLMKPG GTSIVKAAGC MGCWASKSIA MTRVCALPHP AMRAI
//