ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry O31422


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name SKFA_BACSU
Primary accession number O31422
Secondary accession numbers None
Integrated into Swiss-Prot on July 11, 2003
Sequence was last modified on January 1, 1998 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 43)
Name and origin of the protein
Protein name Sporulation-killing factor skfA
Synonyms None
Gene name
Name: skfA
Synonyms: ybcO
OrderedLocusNames: BSU01910
From
Bacillus subtilis [TaxID: 1423] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=168;
Haga K., Liu H., Yasumoto K., Takahashi H., Yoshikawa H.;
"Sequence analysis of the 70kb region between 17 and 23 degree of the Bacillus subtilis chromosome.";
Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=168;
DOI=10.1038/36786; PubMed=9384377 [NCBI, ExPASy, EBI, Israel, Japan]
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.;
"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.";
Nature 390:249-256(1997).
[3]
INHIBITION OF XANTHOMONAS GROWTH.
DOI=10.1046/j.1365-2672.2001.01475.x; PubMed=11851812 [NCBI, ExPASy, EBI, Israel, Japan]
Lin D., Qu L.-J., Gu H., Chen Z.;
"A 3.1-kb genomic fragment of Bacillus subtilis encodes the protein inhibiting growth of Xanthomonas oryzae pv. oryzae.";
J. Appl. Microbiol. 91:1044-1050(2001).
[4]
FUNCTION.
STRAIN=168 / PY79;
DOI=10.1126/science.1086462; PubMed=12817086 [NCBI, ExPASy, EBI, Israel, Japan]
Gonzalez-Pastor J.E., Hobbs E.C., Losick R.;
"Cannibalism by sporulating bacteria.";
Science 301:510-513(2003).
[5]
ROLE IN LIPASE PRODUCTION.
STRAIN=168;
DOI=10.1128/AEM.71.4.1899-1908.2005; PubMed=15812018 [NCBI, ExPASy, EBI, Israel, Japan]
Westers H., Braun P.G., Westers L., Antelmann H., Hecker M., Jongbloed J.D.H., Yoshikawa H., Tanaka T., van Dijl J.M., Quax W.J.;
"Genes involved in SkfA killing factor production protect a Bacillus subtilis lipase against proteolysis.";
Appl. Environ. Microbiol. 71:1899-1908(2005).
[6]
REPRESSION BY ABRB.
DOI=10.1128/JB.187.4.1357-1368.2005; PubMed=15687200 [NCBI, ExPASy, EBI, Israel, Japan]
Fujita M., Gonzalez-Pastor J.E., Losick R.;
"High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis.";
J. Bacteriol. 187:1357-1368(2005).
[7]
INDUCTION.
STRAIN=168;
DOI=10.1128/JB.00084-06; PubMed=16816204 [NCBI, ExPASy, EBI, Israel, Japan]
Allenby N.E.E., Watts C.A., Homuth G., Pragai Z., Wipat A., Ward A.C., Harwood C.R.;
"Phosphate starvation induces the sporulation killing factor of Bacillus subtilis.";
J. Bacteriol. 188:5299-5303(2006).
[8]
REPRESSION BY ABRB AND ABH.
DOI=10.1128/JB.01081-07; PubMed=17720793 [NCBI, ExPASy, EBI, Israel, Japan]
Strauch M.A., Bobay B.G., Cavanagh J., Yao F., Wilson A., Le Breton Y.;
"Abh and AbrB control of Bacillus subtilis antimicrobial gene expression.";
J. Bacteriol. 189:7720-7732(2007).
Comments
  • FUNCTION: Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, providing a source of nutrients to support sporulation. Can also inhibit growth of other bacteria at high concentrations. Has a role in protecting the secreted lipase lipA against proteolysis, either by modulating its folding or by acting as a protease inhibitor.
  • SUBCELLULAR LOCATION: Secreted. Note=Secreted by the ABC transporter skfEF (Probable).
  • INDUCTION: By spo0A and phoP, during nutrient starvation, especially phosphate starvation. Repressed by abrB during normal growth when nutrients are plentiful, in association with the transcriptional repressor abh.
  • WEB RESOURCE: Name=Protein Spotlight; Note=I'll have you for supper - Issue 90 of January 2008; URL="http://www.expasy.org/spotlight/back_issues/sptlt090.shtml";.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AB006424; BAA33086.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z99105; CAB11984.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR C69746; C69746.
RefSeq NP_388072.1; -.
3D structure databases
ModBase O31422.
Enzyme and pathway databases
BioCyc BSUB224308:BSU0191-MON; -.
Organism-specific databases
SubtiList BG12713; skfA. [Micado]
Family and domain databases
BLOCKS O31422.
Genome annotation databases
GeneID 938506; -.
GenomeReviews AL009126_GR; BSU01910.
KEGG bsu:BSU01910; -.
NMPDR fig|224308.1.peg.191; -.
Phylogenomic databases
HOGENOM O31422; -.
Other
LinkHub O31422; -.
Genome annotation databases
CMR O31422; BSU01910.
Other
ProtoNet O31422.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Antibiotic; Antimicrobial; Complete proteome; Secreted.
Features
SEVIEWER logo Feature table viewer
KeyFrom To Length Description FTId
CHAIN   1   55  55     Sporulation-killing factor skfA. PRO_0000049459
Sequence information
Length: 55 AA [This is the length of the unprocessed precursor] Molecular weight: 5934 Da [This is the MW of the unprocessed precursor] CRC64: F169E627ADBEBB55 [This is a checksum on the sequence]
        10         20         30         40         50 
MKRNQKEWES VSKKGLMKPG GTSIVKAAGC MGCWASKSIA MTRVCALPHP AMRAI 

O31422 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by ca flag CBR Canada Mirror sites: Australia  Brazil  China  Korea  Switzerland
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!