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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: O03042




SWISS-2DPAGE:  O03042


O03042


General information about the entry
View entry in simple text format
Entry nameRBL_ARATH
Primary accession numberO03042
integrated into SWISS-2DPAGE on December 1, 2000 (release 13)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on January 31, 2008 (version 6)
Name and origin of the protein
DescriptionRibulose bisphosphate carboxylase large chain (EC 4.1.1.39) (RuBisCO large subunit).
Gene nameName=rbcL
OrderedLocusNames=AtCg00490
Annotated speciesArabidopsis thaliana (Mouse-ear cress) [TaxID: 3702]
TaxonomyEukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
References
[1]   MAPPING ON GEL
Sarazin B., Tonella L., Marques K., Paesano S., Chane-Favre L., Sanchez J.-C., Hochstrasser D.F., Thiellement H.
Submitted (OCT-2000) to the SWISS-2DPAGE database
[2]   MAPPING ON GEL
MEDLINE=99155804; PubMed=10036779; [NCBI, ExPASy, EBI, Israel, Japan]
Santoni V., Rouquie D., Doumas P., Mansion M., Boutry M., Degand H., Dupree P., Packman L., Sherrier J., Prime T., Bauw G., Posada E., Rouze P., Dehais P., Sahnoun I., Barlier I., Rossignol M.
''Use of a proteome strategy for tagging proteins present at the plasma membrane''
Plant J. 16:633-641(1998)
2D PAGE maps for identified proteins
How to interpret a protein

ARABIDOPSIS {Arabidopsis thaliana}
Arabidopsis thaliana (Mouse-ear cress)
Tissue: Leaf
ARABIDOPSIS
  map experimental info
  protein estimated location
 
ARABIDOPSIS

MAP LOCATIONS:
pI=5.71; Mw=50864  [identification data]
pI=5.64; Mw=51074  [identification data]
pI=5.78; Mw=51284  [identification data]
pI=6.15; Mw=47034  [identification data]
pI=6.10; Mw=43852  [identification data]
pI=6.02; Mw=33897  [identification data]
pI=5.92; Mw=32050  [identification data]
pI=5.55; Mw=30287  [identification data]
pI=5.79; Mw=29705  [identification data]
pI=5.79; Mw=29370  [identification data]

MAPPING (identification):
MASS SPECTROMETRY [2];
SPOT 2D-001K6O: GEL MATCHING [1] WITH 2-D MAP OF LEAF SOLUBLE PROTEINS FROM PPMdb DATABASE (GHENT, BELGIUM) [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://www.expasy.org/ch2d/license.html or send email to license@isb-sib.ch).
Cross-references
UniProtKB/Swiss-ProtO03042; RBL_ARATH.
2D PAGE maps for unidentified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.
Dictyostelium discoideum
(Slime mold)
Dictyostelium discoideum
DICTYSLUG
Escherichia coli
Escherichia coli
ECOLI
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5
Escherichia coli(4-5)
ECOLI4-5
Escherichia coli(4.5-5.5)
ECOLI4.5-5.5
Escherichia coli(5-6)
ECOLI5-6
Escherichia coli(5.5-6.7)
ECOLI5.5-6.7
Escherichia coli(6-11)
ECOLI6-11
Escherichia coli(6-9)
ECOLI6-9
Homo sapiens
(Human)
Colorectal epithelia cells
CEC_HUMAN
Cerebrospinal Fluid
CSF_HUMAN
Colorectal adenocarcinoma cell line (DL-1)
DLD1_HUMAN
Erythroleukemia Cell
ELC_HUMAN
HepG2 Secreted Proteins
HEPG2SP_HUMAN
HepG2
HEPG2_HUMAN
Promyelocytic leukemia cells
HL60_HUMAN
Kidney
KIDNEY_HUMAN
Liver
LIVER_HUMAN
Lymphocytes
LYMPHOCYTE_HUMAN
Lymphoma
LYMPHOMA_HUMAN
Soluble nuclear proteins and matrix from liver tissue
NUCLEI_LIVER_HUMAN
SDS-PAGE of nucleolar proteins from Human HeLa cells
NUCLEOLI_HELA_1D_HUMAN
2D-PAGE of nucleolar proteins from Human HeLa cells
NUCLEOLI_HELA_2D_HUMAN
Plasma
PLASMA_HUMAN
Platelet
PLATELET_HUMAN
Red blood cells
RBC_HUMAN
Macrophage Like Cell Line
U937_HUMAN
Mus musculus
(Mouse)
Brown adipose tissue
BAT_MOUSE
Pancreatic islet cells
ISLETS_MOUSE
Liver
LIVER_MOUSE
Gastrocnemius muscle
MUSCLE_MOUSE
Soluble nuclear proteins and matrix from liver tissue
NUCLEI_LIVER_MOUSE
White adipose tissue
WAT_MOUSE
Saccharomyces cerevisiae
(Baker's yeast)
Saccharomyces cerevisiae
YEAST
Staphylococcus aureus
(strain N315)
Staphylococcus aureus
STAPHYLOCOCCUS



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 54.70
Entry nameRBL_ARATH
Primary accession numberO03042
Name and origin of the protein
DescriptionRibulose bisphosphate carboxylase large chain precursor (EC 4.1.1.39) (RuBisCO large subunit)
Gene nameName=rbcL
OrderedLocusNames=AtCg00490
KeywordsAcetylation; Calvin cycle; Carbon dioxide fixation; Chloroplast; Complete proteome; Lyase; Magnesium; Metal-binding; Methylation; Monooxygenase; Oxidoreductase; Photorespiration; Photosynthesis; Plastid.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLU91966; AAB68400.1; -; Genomic_DNA.
EMBLAP000423; BAA84393.1; -; Genomic_DNA.
EMBLAB003522; BAA20946.1; -; Genomic_DNA.
EMBLD88901; BAA19595.1; -; Genomic_DNA.
RefSeqNP_051067.1; -.
HSSPP00880; 1RBL.
SMRO03042; 11-475.
SWISS-2DPAGEO03042; ARATH.
GeneID844754; -.
GenomeReviewsAP000423_GR; ATCG00490.
TAIRAtCg00490; -.
GermOnlineATCG00490; Arabidopsis thaliana.
GOGO:0016984; F:ribulose-bisphosphate carboxylase activity; IEA:HAMAP.
HAMAPMF_01338; -; 1.
InterProIPR000685; RuBisCO_large.
Gene3DG3DSA:3.20.20.110; RuBisCO_large; 1.
Gene3DG3DSA:3.30.70.150; RuBisCO_large; 1.
PfamPF00016; RuBisCO_large; 1.
PfamPF02788; RuBisCO_large_N; 1.
PROSITEPS00157; RUBISCO_LARGE; 1.




Database constructed and maintained by Christine Hoogland, using the Make2D-DB II package (ver. 2.50.2) from the World-2DPAGE Constellation of the ExPASy web server

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