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ENZYME

ENZYME entry: EC 2.3.2.31

Accepted Name
RBR-type E3 ubiquitin transferase
Reaction catalysed
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine <=> [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine
Comment(s)
  • RBR-type E3 ubiquitin transferases have two RING fingers separated by an internal motif (IBR, for In Between RING).
  • The enzyme interacts with the CRL (Cullin-RING ubiquitin Ligase) complexes formed by certain RING-type E3 ubiquitin transferase (see EC 2.3.2.27), which include a neddylated cullin scaffold protein and a substrate recognition module.
  • The RING1 domain binds an EC 2.3.2.23, and transfers the ubiquitin that is bound to it to an internal cysteine residue in the RING2 domain, followed by the transfer of the ubiquitin from RING2 to the substrate.
  • Once the substrate has been ubiquitinated by the RBR-type ligase, it can be ubiqutylated further using ubiquitin carried directly on E2 enzymes, in a reaction catalyzed by EC 2.3.2.27.
  • Activity of the RBR-type enzyme is dependent on neddylation of the cullin protein in the CRL complex. cf. EC 2.3.2.26, EC 2.3.2.27, and EC 2.3.2.32.
Cross-references
BRENDA2.3.2.31
EC2PDB2.3.2.31
ExplorEnz2.3.2.31
PRIAM enzyme-specific profiles2.3.2.31
KEGG Ligand Database for Enzyme Nomenclature2.3.2.31
IUBMB Enzyme Nomenclature2.3.2.31
IntEnz2.3.2.31
MEDLINEFind literature relating to 2.3.2.31
MetaCyc2.3.2.31
Rhea expert-curated reactions2.3.2.31
UniProtKB/Swiss-Prot
Q1L8G6, AKIB1_DANREQ9P2G1, AKIB1_HUMANQ6ZPS6, AKIB1_MOUSE
Q9SKC4, ARI10_ARATHQ9SKC2, ARI11_ARATHO01965, ARI11_CAEEL
Q84RQ9, ARI12_ARATHQ9FFN9, ARI13_ARATHQ9FFP1, ARI14_ARATH
Q84RQ8, ARI15_ARATHQ9C5A4, ARI16_ARATHQ6NW85, ARI1L_DANRE
Q949V6, ARI1_ARATHA2VEA3, ARI1_BOVINQ6PFJ9, ARI1_DANRE
Q94981, ARI1_DROMEQ9Y4X5, ARI1_HUMANQ9Z1K5, ARI1_MOUSE
Q32NS4, ARI1_XENLAB1H1E4, ARI1_XENTRQ84RR2, ARI2_ARATH
Q22431, ARI2_CAEELO76924, ARI2_DROMEO95376, ARI2_HUMAN
Q9Z1K6, ARI2_MOUSEQ9LVX0, ARI3_ARATHQ9LVW9, ARI4_ARATH
Q8L829, ARI5_ARATHP0C8K8, ARI6_ARATHQ84RR0, ARI7_ARATH
Q8W468, ARI8_ARATHQ9SKC3, ARI9_ARATHQ9P3U4, HEL1_SCHPO
P36113, HEL1_YEASTA9JTG5, HOIL1_DANREE6ZIJ1, HOIL1_DICLA
Q9BYM8, HOIL1_HUMANQ9WUB0, HOIL1_MOUSEQ62921, HOIL1_RAT
Q9US46, ITT1_SCHPOQ04638, ITT1_YEASTQ8IPJ3, LUBEL_DROME
Q7KTX7, PRKN_DROMEO60260, PRKN_HUMANQ9WVS6, PRKN_MOUSE
E0VIU9, PRKN_PEDHCQ9JK66, PRKN_RATQ5RFV4, R1441_DANRE
Q6DH94, R1442_DANREP50876, R144A_HUMANQ925F3, R144A_MOUSE
A4IIY1, R144A_XENTRA5PK27, R144B_BOVINQ7Z419, R144B_HUMAN
Q8BKD6, R144B_MOUSEQ6T486, RBRA_DICDIF4ITM1, RGSL1_ARATH
Q9NV58, RN19A_HUMANP50636, RN19A_MOUSEQ2VJ60, RN19A_PIG
Q1L8L6, RN19B_DANREQ6ZMZ0, RN19B_HUMANA2A7Q9, RN19B_MOUSE
Q08B84, RN19B_XENLAQ8TC41, RN217_HUMAND3YYI7, RN217_MOUSE
Q4KLT0, RN217_XENLAF1RB95, RNF14_DANREQ9UBS8, RNF14_HUMAN
Q9JI90, RNF14_MOUSEQ96EP0, RNF31_HUMANQ924T7, RNF31_MOUSE
Q54CX4, Y5521_DICDI

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